Culturing Porphyridium purpureum - ALG App005

  • By algenuity
  • 12 Jul, 2016

Background to P. purpureum

P. purpureum is a unicellular red microalga which is found in most terrestrial areas including on submerged river banks and saltmarshes; it is even found within brickwork. Commercial relevance of P. purpureum stems from its high protein content and presence of value-added products including high value polysaccharides, arachidonic acid (ARA) and phycoerythrin (Juin et al., 2015; Su et al., 2016). Its genome was sequenced in 2013 (Bhattacharya et al., 2013). Growth rates and levels of polysaccharides have been found to correlate with light intensity (Valea et al., 2011); however, the effect of light colour on P. purpureum growth is yet to be reported.

Aim

To determine the impact of light colour on  Porphyridi um purpureum  growth. 

Experimental Design

An exponentially growing culture of P. purpureum in late-log phase was inoculated at 1 x 105 cells/ml into 400 ml DM04 medium (in-house phototrophic marine medium). Cultures were grown in the Algem at 22°C under 240 μmol photons/m²/s continuous light consisting of either red (660nm), blue (465nm), or simulated sunlight (a 85:15 white/red mix). Cultures were mixed at 120 rpm with 5 cm³/min aeration of 5 % CO₂ in air. Experiments were conducted in duplicate, with growth assessed by in-flask optical density (OD) measurements at 740nm.
Wavelength graphs of sunlight, and red and blue light
Figure 1 – Spectra of light under investigation

Results

Growth curves of algae cultures growing on three different  light profiles
Figure 2 - Growth profiles of P. purpureum cultured photoautotrophically in DM04 medium under different light colours
(sunlight, red, and blue) (n = 2)
Six flasks of red algae cultures
Figure 3 - Culture health after 7 days in the Algem; from left to right sunlight, red, and blue

Discussion

P. purpureum was observed to grow significantly better under simulated sun- and red light compared to blue light as measured by OD 740nm accumulation (Figure 2). A dense, homogenous culture without clumping was observed for all conditions with noticeably lower biomass for blue light illuminated flasks. Further experiments should be conducted in the Algem to further optimise light intensity and temperature for optimal growth.

References

Bhattacharya, D., Price, D.C., Chan, C.X., Qiu, H., Rose, N., Ball, S., Weber, A.P., Arias, M.C., Henrissat, B., Coutinho, P.M. and Krishnan, A. (2013) Genome of the red alga Porphyridium purpureum. Nature communications, 4, pp. 1-10

Juin, C., Chérouvrier, J.R., Thiéry, V., Gagez, A.L., Bérard, J.B., Joguet, N., Kaas, R., Cadoret, J.P. and Picot, L. (2015) Microwave-assisted extraction of phycobiliproteins from Porphyridium purpureum. Applied Biochemistry and Biotechnology , 175 (1), pp.1-15.

Su, G., Jiao, K., Li, Z., Guo, X., Chang, J., Ndikubwimana, T., Sun, Y., Zeng, X., Lu, Y. and Lin, L. (2016) Phosphate limitation promotes unsaturated fatty acids and arachidonic acid biosynthesis by microalgae Porphyridium purpureum . Bioprocess and Biosystems Engineering , pp.1-8

Velea, S., Ilie, L. and Filipescu, L. (2011) Optimization of Porphyridium purpureum culture growth using two variables
experimental design: light and sodium bicarbonate. UPB Science Bulletin Series B, 73 (4), pp.81-94

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